They are all "worthwhile"--I typically decide based on airfare and personal interest. They are three of the most famous world cities for a reason!

Can you go to all three of them? Or you only want to go one place?

They are all "worthwhile".

Personally I'd go with Barcelona or Florence, but that's because I've already been to Amsterdam.

One of my favorite cities is Barcelona...look into Lisbon also. The previous commenter was right, they are all worth your time.

Amsterdam.

No one can decide for you - but maybe give us more information.

Time of year? That could make a BIG difference. If I had a choice, wouldn't go to Florence in the hottest summer months nor Amsterdam in the coldest winter months. In the Spring all three would be great but the flowers in Netherlands are Awesome.

Which has the cheapest flights from your home town?

What are you interested in?

Hi,
I would pick Amsterdam. If you are staying in 1 city, that would be my choice. There are many day trips you can take when you are based in Amsterdam.
And I am not sure how long you are staying, but maybe you can add a few days in Paris to your experience? It's only a 3.5 hour by train.

I would NOT pick Amsterdam, precisely because of the bike culture, which makes an already overpriced city with mediocre food terribly annoying IMO. I would pick Paris or Rome. Both have ample daytrip opportunities, particularly Paris.

It really depends on your interest they are all so unique. I would choose Florence as it is more quintessentially "European" to me out of the three but again, they are all wonderful in their own ways. So, choose whichever is more convenient to travel to and you won't be disappointed.

And see, I would NEVER pick Florence, which doesn't seem terribly "European" to me at all, and which is too frenetic for me and overpriced and filled with religious art, which totally turns my stomach. When I think of "totally European" I think of places like Narbonne, which have been humming along nicely for a few thousand years without attracting people in shorts and flip-flops and which have gobs of treasures to show for it.

But I agree with deniseoliver86 that wherever you choose will likely delight you.

First time hearing the name Narbonne, will deffo look into it! Thanks StCirq

You don't think Paris or Rome are overpriced. But true, any big tourist city you go to you will be paying a "tourist" price. Maybe look into one country that you like and see if there are some smaller towns that can be interesting. Europe has many countries with completely different cultures.
And I am sure that wherever you go, you will have a good time.

No, I do not think Paris or Rome is overpriced. But I know my way around them pretty well and know how not to pay "tourist prices," whatever those are. You can go almost anywhere in the world and set a budget and live within in.

Why do you want to choose only between these 3?

If this is your first time in Europe every European city will be new for you.

Europe has hundreds of great cities.

I would not recommend Amsterdam, Barcelona and Florence. All 3 are suffering from overtourism. More tourists is the last thing they need.

Amsterdam does have wonderful museums and the canals are enticing, but some of the worst food in western Europe.

Barcelona is indeed unique but it is being ruined by people like us-tourist.

I will be at the airport at Florence Tuesday and will drive past the city. We have been there a number of times and in the center you will hear very little Italian.

You need to educate yourself of what each offers and their disadvantages.

My choices for a first timer to Europe would be London, Paris, Rome.