Trains on Italy's main north–south rail line stop in Florence as well as Prato, Arezzo, and Chiusi. Another major line connects Florence with Pisa, and the coastal line between Rome and Genoa passes through Pisa as well. There's regular, nearly hourly service from Florence to Lucca, and several trips a day between Florence and Siena. Siena's train station is 2 km (1 mile) north of the centro storico (historic center), but cabs and city buses are readily available, as is a very handy funicular.
For other parts of Tuscany—Chianti, Montalcino, and Montepulciano, for example—you're better off traveling by bus or by car. Train stations, when they exist, are far from the historic centers (usually in the valleys below hill towns), and service is infrequent.
Trenitalia. You can check timetables on the website of the Italian state railway system and also get information and tickets at most travel agencies. 892021; www.trenitalia.com.