This way-off-the-beaten-path museum (the name translates as the Museum of the Last Supper) has a stunning fresco by Andrea del Sarto. Begun sometime around 1511 and finished in 1526–27, the fresco depicts the moment when Christ announced that one of his apostles would betray him. Andrea has rendered the scene in subtle yet still brilliant colors. Also on display are a couple of lesser-known works by Pontormo and copies of other 16th-century works. (Down the street is the church of San Salvi, founded by John Gualbert and begun in 1048. Though it suffered damage during the siege of 1529–30, the interior has a modest but lovely Madonna and Child by Lorenzo di Bicci as well as a 16th-century wood cross on the altar.) To get here, take Bus 6 from Piazza San Marco and get off at the Lungo L'Affrico stop—it's the first stop after crossing the railroad tracks.
Via San Salvi 16, Florence, 50121, Italy