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Florence to Rome with a stop in Assisi

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Hello! I have about 2 weeks in Italy. I will be staying some days in Florence and would like to visit Assisi. Is it possible to go from Florence early morning to Assisi and then to Rome in the evening the same day?

If so, what would I do with my luggage once I get to Assisi?

I can always stay a night in Assisi, but I am trying to avoid the hassle of checking in and out of another hotel.

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